American Society of Hematology

Fig. 1.

$Fig. 1. Identification of myeloid progenitors in murine fetal liver. / (A) Live, Lin−IL-7R−AA4.1− cells were gated and analyzed for expression of the c-Kit and Sca-1 surface markers. The Lin−IL-7R−AA4.1−Sca-1−c-Kit+fraction was subdivided into FcγRloCD34+, FcγRloCD34−, and FcγRhiCD34+ populations. Percentages of each population relative to whole FL are shown next to each sort gate. (B) Reanalysis of sorted populations. Each was isolatable to purity after 2 consecutive sorts. (C) Cellular morphology of sorted populations (60 × magnification, Giemsa/May-Grünwald staining). Note the unusual cytoplasmic protrusions of FcγRloCD34− cells and the presence of myelomonocytic characteristics in FcγRhiCD34+cells.$

Identification of myeloid progenitors in murine fetal liver.

(A) Live, Lin⁻IL-7R⁻AA4.1⁻ cells were gated and analyzed for expression of the c-Kit and Sca-1 surface markers. The Lin⁻IL-7R⁻AA4.1⁻Sca-1⁻c-Kit⁺fraction was subdivided into FcγR^loCD34⁺, FcγR^loCD34⁻, and FcγR^hiCD34⁺ populations. Percentages of each population relative to whole FL are shown next to each sort gate. (B) Reanalysis of sorted populations. Each was isolatable to purity after 2 consecutive sorts. (C) Cellular morphology of sorted populations (60 × magnification, Giemsa/May-Grünwald staining). Note the unusual cytoplasmic protrusions of FcγR^loCD34⁻ cells and the presence of myelomonocytic characteristics in FcγR^hiCD34⁺cells.

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