Expression of transcripts for MBP in MCs and eosinophils with RT-PCR.

The transcripts for MBP (440 bp) and those for β-actin (661 bp) are shown in lanes 1 to 4 and lanes 5 to 8, respectively. The transcripts were obtained from PB-derived MCs (lanes 1, 5), CB-derived MCs (lanes 2, 6), PB-derived primary eosinophils (lanes 3, 7), and CB-derived immature cultured eosinophils (lanes 4, 8).26,27Similar results were obtained with 2 other experiments.