Fig. 12.
Fig. 12. Stem cell toxic effect of low-dose irradiation on donor stem cells. / Lin− Hoechst 33342lowRhodamine123low–sorted stem cells from male BALB/c mice (1.8 × 104 per mouse) were injected intravenously in 1 Gy–treated female BALB/c recipients. Prior to transplantation, half of the sorted stem cells were exposed to 1 Gy irradiation, and the other half received no treatment. Engraftment was measured in bone marrow 8 weeks after transplantation with FISH using a Y-chromosome painting probe. Cells were counted using a Rhodamine filter to give the percentage of engraftment. Mean ± SE is shown (n = 5,P ≤ .001).

Stem cell toxic effect of low-dose irradiation on donor stem cells.

Lin− Hoechst 33342lowRhodamine123low–sorted stem cells from male BALB/c mice (1.8 × 104 per mouse) were injected intravenously in 1 Gy–treated female BALB/c recipients. Prior to transplantation, half of the sorted stem cells were exposed to 1 Gy irradiation, and the other half received no treatment. Engraftment was measured in bone marrow 8 weeks after transplantation with FISH using a Y-chromosome painting probe. Cells were counted using a Rhodamine filter to give the percentage of engraftment. Mean ± SE is shown (n = 5,P ≤ .001).

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