Relation between ATM levels and p53 dysfunction.

ATM and β-actin levels were measured by Western blot analysis in 31 patients with CLL. Normal and A-T lymphoblastoid cell lines (LCL) were used as positive and negative controls, respectively. β-Actin and central ATM bands were quantified by densitometry, and the ATM:β-actin ratio of each lane was calculated. To enable comparisons to be made between individual membranes, the ATM:β-actin ratios were normalized with respect to the control cells on the same membrane. Dagger and asterisk denote patients with types A (patients 9, 10, 11, 12) and B (patients 6, 8, 19, 24) p53 dysfunction, respectively. The Figure shows the results obtained using the FP8r antibody, but similar results were obtained with AHP397.