Fig. 7.
Fig. 7. Proliferative indices of common myeloid progenitors. / CMPs were purified by flow cytometry, and proliferation was measured by BrdU incorporation and PCNA staining of cytospin preparations. ForPbx1+/− CMPs, 40% of cells that stained with propidium iodide showed incorporation of BrdU into their DNA during the 90-minute labeling period, and 80% showed nuclear punctate PCNA staining. Equivalent staining of Pbx1−/− CMPs was 20% for BrdU and 25% for PCNA.

Proliferative indices of common myeloid progenitors.

CMPs were purified by flow cytometry, and proliferation was measured by BrdU incorporation and PCNA staining of cytospin preparations. ForPbx1+/−CMPs, 40% of cells that stained with propidium iodide showed incorporation of BrdU into their DNA during the 90-minute labeling period, and 80% showed nuclear punctate PCNA staining. Equivalent staining of Pbx1−/−CMPs was 20% for BrdU and 25% for PCNA.

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