Phenotypic characterization of reconstituted BM chimeras.

Three months after BM reconstitution, mice from either H-2^d→H-2^dxb (B) or H-2^b→H-2^dxb chimeras (C) were killed. Splenocytes from these animals were stained for the expression of I-E^d using the MoAb 14.4.4 (IgG2a), or for the expression of I-A^b with the MoAb Y3P (IgG2a). FITC goat anti–mouse IgG2a secondary antibody was added and the stained splenocytes were analyzed for MHC class II expression by FACS. Splenocytes from an H-2^bxd mouse (A) were used as a positive control. Shaded histogram in each figure represents background staining.