Functional characterization of patient PBMCs circulating at day +45 after PBSC transplantation (PBMCs–ex vivo).

Effector cells were used ex vivo without further in vitro resimulation and tested at various effector-target ratios in a standard 4-hour chromium release assay using donor BLCLs, patient BLCLs, or the allogeneic BLCL OMW as targets. The latter 2 BLCLs were used untransduced (○) or transduced (●) with retroviral vector LDPβ9SΔN encoding HLA-DPβ1*0901. For complete HLA typing of BLCLs, see Table 1. Values are means of duplicates, with error bars indicating SDs.