![Fig. 3. Expression of LRP protein in isolated nuclei and accumulation and efflux of DOX. / (A) Immunoblot analysis. The expression levels of LRP in isolated nuclei. (B) The levels of [14C]DOX in the isolated nuclei after incubation without (▪) or with (■) anti-LRP antibody were determined. Values represent means ± SEs of 3 separate experiments. *P < .05 (C) Efflux of [14C]DOX. [14C]DOX retained in nuclei isolated from KUT-2 cells without (●) or with (○) treatment with anti-LRP antibody; ATL cells from patient 29 without (▴) or with (▵) treatment with anti-LRP antibody; and ATL cells from patient 31 without (▪) or with (■) treatment with anti-LRP antibody. The effluxes of [14C]DOX from the nuclei isolated from ATL samples (patients 29 and 31) were significantly enhanced compared with nuclei from KUT-2 cells (P < .05). The anti-LRP antibody partially inhibited the enhanced efflux from the nuclei isolated from the ATL cells (patients 29 and 31) (P < .05). Values represent means ± SEs of 3 separate experiments. (D) The accumulation of DOX in the isolated nuclei from KUT-2 and from patients 28, 29, 31, and 32 was assessed by fluorescence microscopy. DPM indicates decay per minute.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/4/10.1182_blood.v98.4.1160/6/h81611381003.jpeg?Expires=1726834052&Signature=XdBKqwgbIp366M4y1z5-UY0QjBJd6-vbBX6zI1yUhg5ZaXhZOkv7OCi5IGR~sd4oDNKZPiA2HQe7E3dBbAB4~B3KrePBaULu5HKlZWykPxYXhFUZBUdAE61SpodrasxePJbMk9AfOTMkOds12-xSpwN-Occ6XAVFman-HXbnY9nmG74TE5N9UDtJNJQDdn2Ey5raWy-qGZ5XFIaQ8MMAyi7d7Jy1~9QJkp25DDIgQ2SrYkfYBZKcBNAKVMZCgJckCGjFlO~FgFioZ35MB8aw9YGW1JnaMZek7~mVi1lCt7DnHPMAvs1W8otQa8YfjAXTD0GD0X5NLfFDvt9l9SxmgA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Expression of LRP protein in isolated nuclei and accumulation and efflux of DOX.
(A) Immunoblot analysis. The expression levels of LRP in isolated nuclei. (B) The levels of [14C]DOX in the isolated nuclei after incubation without (▪) or with (■) anti-LRP antibody were determined. Values represent means ± SEs of 3 separate experiments. *P < .05 (C) Efflux of [14C]DOX. [14C]DOX retained in nuclei isolated from KUT-2 cells without (●) or with (○) treatment with anti-LRP antibody; ATL cells from patient 29 without (▴) or with (▵) treatment with anti-LRP antibody; and ATL cells from patient 31 without (▪) or with (■) treatment with anti-LRP antibody. The effluxes of [14C]DOX from the nuclei isolated from ATL samples (patients 29 and 31) were significantly enhanced compared with nuclei from KUT-2 cells (P < .05). The anti-LRP antibody partially inhibited the enhanced efflux from the nuclei isolated from the ATL cells (patients 29 and 31) (P < .05). Values represent means ± SEs of 3 separate experiments. (D) The accumulation of DOX in the isolated nuclei from KUT-2 and from patients 28, 29, 31, and 32 was assessed by fluorescence microscopy. DPM indicates decay per minute.
