NF-κB inhibitors induce apoptosis in Tax transgenic tumor cells.
(A) Fresh tumor and spleen cell suspensions from nontransgenic (NT) and Tax transgenic mice were incubated in the presence (+) or absence (−) of 10 mM sodium salicylate (SA) for 20 hours prior to treatment with 20 Gy (2000 rad) irradiation. Five hours post-irradiation, 105cells were stained with fluorescein isothiocyanate-conjugated antibodies against annexin V (FL1-H) and propidium iodide (PI, FL2-H). Late-stage apoptotic cells are indicated by dual positivity with annexin V and PI, and the percentage of positive cells in each quadrant is shown. (B) Fresh tumor and spleen cell suspensions from nontransgenic (NT) and Tax transgenic mice were incubated in the presence or absence of 10 μM or 20 μM prostaglandin A1 (PGA1) or 15-deoxy-Δ12,14-prostaglandin J2 (15dPGJ2) for 16 hours prior to annexin V and propidium iodide staining. Early-stage apoptotic cells are indicated by annexin V single positive staining, and the percentage of positive cells in each quadrant is shown.
