Sodium salicylate and cyclopentenone prostaglandins inhibit spontaneous proliferation of Tax transgenic mouse splenocytes.

(A) A total of 10⁷ spleen cells from NT and Tax transgenic mice were cultured for 4 hours following sodium salicylate or mock treatment. NT splenocytes were also stimulated with 500 U/mL IL-2 plus 10 μg/mL PHA. Then, 1.1 MBq per well of [³H]thymidine was added to cultures, and thymidine incorporation was measured after a 16-hour incubation. (B) The percent cell viability was determined by trypan blue exclusion prior to cell harvest. (C) A total of 10⁷ spleen cells from NT and Tax transgenic mice were cultured for 2 hours following PGA₁, 15dPGJ₂, or mock treatment. NT splenocytes were also stimulated with 500 U/mL IL-2 plus 10 μg/mL PHA. [³H]thymidine was added to cultures, and thymidine incorporation was measured 14 hours later. All experiments were performed in triplicate, and error bars represent the percent SE.