Fig. 6.
Fig. 6. Cytolytic assays of expanded CTLs. / A rapidly expanded pp65495-503-specific CTL line shows specific killing of (A) autologous PHA-blasts pulsed with pp65495-503 (▪) and (B) the CMV-infected HLA-*A0201+ MRC5 fibroblast cell line (♦). The specific lysis of the PHA-blasts is higher because they will present more exogenous peptide at the cell surface compared to the CMV-infected MRC5 fibroblast cell line, which presents endogenously processed, pp65-derived peptide. Negative controls were not killed: ♦, PHA blasts plus E711-20; ●, K562; and ▴, noninfected MRC-5 cells.

Cytolytic assays of expanded CTLs.

A rapidly expanded pp65495-503-specific CTL line shows specific killing of (A) autologous PHA-blasts pulsed with pp65495-503 (▪) and (B) the CMV-infected HLA-*A0201+ MRC5 fibroblast cell line (♦). The specific lysis of the PHA-blasts is higher because they will present more exogenous peptide at the cell surface compared to the CMV-infected MRC5 fibroblast cell line, which presents endogenously processed, pp65-derived peptide. Negative controls were not killed: ♦, PHA blasts plus E711-20; ●, K562; and ▴, noninfected MRC-5 cells.

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