Fig. 2.
Fig. 2. T-cell proliferation assays. / (A) Mature DCs grown in either FBS or AP are equally efficient at inducing allogeneic T-cell proliferation. (B) FBS-DCs induce autologous T-cell proliferation due to recognition of FBS-derived peptides. Primary responses were stimulated from autologous T cells by FBS-DCs with KLH added (●) and in the FBS-DC autologous MLR without antigen (▴) suggesting that xenogeneic FBS peptides are presented to autologous T cells. In contrast, the AP-DCs stimulated a response only when the foreign KLH antigen was present (▪), versus without KLH (♦).

T-cell proliferation assays.

(A) Mature DCs grown in either FBS or AP are equally efficient at inducing allogeneic T-cell proliferation. (B) FBS-DCs induce autologous T-cell proliferation due to recognition of FBS-derived peptides. Primary responses were stimulated from autologous T cells by FBS-DCs with KLH added (●) and in the FBS-DC autologous MLR without antigen (▴) suggesting that xenogeneic FBS peptides are presented to autologous T cells. In contrast, the AP-DCs stimulated a response only when the foreign KLH antigen was present (▪), versus without KLH (♦).

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