Schematic representation of generation of pp65_495-503-specific CTL lines.

Mature DCs are cultured from CD14⁺ monocytes and used for primary stimulation of CD8⁺ cells. Peptide-pulsed LCLs or CD40-ligated B cells are used for restimulation. The CTL line is further purified with anti-PE–coated paramagnetic beads or flow sorting prior to rapid expansion.