Effects of 5-FU.
Shown are effects of intraperitoneal administration of 150 mg/kg 5-FU (on day 1) on levels of circulating platelets (A), hematocrit (B), and white blood cell counts (C) in wild-type C57/Bl6 mice (▵) and c-mpl−/− mice (●). Baseline blood cell counts were obtained before injection of 5-FU. C-mpl−/− mice had delayed development of thrombocytosis, which did not reach the level observed in wild-type mice. Data were derived from 5 independent experiments and based on a total of 83 c-mpl−/− mice and 31 C57/Bl6 mice. One day before administration of 5-FU and 7 days afterward, blood samples were obtained from all mice, thereby allowing determination of baseline platelet counts and confirmation of the response to 5-FU. Thereafter, blood samples were obtained from groups of 2 to 10 mice. Normal mean ± SE values (n = 17) were platelets, 1053 × 109/L ± 176 × 109/L; hematocrit, 0.47 ± 0.2; and white blood cells, 4.3 ± 2.0 × 109/L.
