Effect of FMF-associated mutations on the ability of pyrin to localize with microtubules.

HeLa cells transfected with GFP-pyrin plasmid encoding 3 of the most common FMF mutations were treated with 10 μM paclitaxel before fixing. (A,D,G,J) GFP-pyrin; (B,E,H,K) staining with mouse anti–β-tubulin and Cy3-conjugated goat anti–mouse IgG; (C,F,I,L) merged images of panels A and B, D and E, G and H, and J and K, respectively. (A-C) Cells transfected with wild-type pyrin; (D-F) pyrin with M680I mutation; (G-I) pyrin with M694V mutation; (J-L) pyrin with V726A mutation. Magnification × 1000.