GFP-pyrin localizes with microtubules in untreated and paclitaxel-treated cells.

HeLa cells were transfected with pEGFP-MEFV.N plasmid and immunostained with anti–β-tubulin and Cy3 conjugated goat anti–mouse IgG. (A) A transfected cell without paclitaxel treatment has a filamentlike GFP pattern. (B) Microtubule staining of the same cell. (C) Merge of GFP and microtubule staining demonstrating colocalization of GFP and microtubule staining. Arrows indicate regions where staining overlap is particularly noticeable. (D) A transfected cell with strong perinuclear GFP signal. (E) GFP-pyrin and (F) microtubule staining of a paclitaxel-treated transfected cell in which colocalization is extensive. Magnification × 1000.