Cytoplasmic localization of full-length pyrin in HeLa cells by confocal microscopy.

HeLa cells transfected with myc-tagged pyrin plasmid were labeled with anti-myc antibody followed by anti–mouse IgG Alex Fluor 488 (green), plus TO-PRO-3 nuclear dye (blue). (A) Proximal XY-plane (0 μM) of immunofluorescence of myc-tagged pyrin. (B) Median XY-plane (−1.05 μm). (C) Distal XY-plane (−2.1 μm). (D) Computer-generated XZ-sections of the same cell. Sections progress from top to bottom of XY-plane images. Magnification × 1000.