Fig. 2.
Fig. 2. Overexpression of the chemokine I-309 in ATL-derived cells. / (A) Total RNA derived from ATL cultures (JuanaW, Champ, PaBe) and HTLV-1− reference cells (PBMCPHA, HuT-78, Jurkat) was subjected to Northern blot analysis using an I-309–specific cDNA probe. The top panel shows a representative autoradiograph. The 550-base RNA band was quantified by phosphorimaging. The lower panel depicts the mean RNA levels ± SD resulting from 3 independent experiments. (B) Culture supernatant from ATL cultures (JuanaW, Champ, PaBe, StEd) and reference cells (TAXI-1, Mondi, MT-2, Jurkat) were subjected to antigen capture ELISA. Plates were coated with monoclonal anti–I-309 antibodies and incubated with serial dilutions of the supernatants. Bound I-309 was detected with biotinylated secondary I-309 antibodies. I-309 concentrations were determined using recombinant human I-309 as a standard. The columns show the mean ± SD of 3 independent experiments.

Overexpression of the chemokine I-309 in ATL-derived cells.

(A) Total RNA derived from ATL cultures (JuanaW, Champ, PaBe) and HTLV-1− reference cells (PBMCPHA, HuT-78, Jurkat) was subjected to Northern blot analysis using an I-309–specific cDNA probe. The top panel shows a representative autoradiograph. The 550-base RNA band was quantified by phosphorimaging. The lower panel depicts the mean RNA levels ± SD resulting from 3 independent experiments. (B) Culture supernatant from ATL cultures (JuanaW, Champ, PaBe, StEd) and reference cells (TAXI-1, Mondi, MT-2, Jurkat) were subjected to antigen capture ELISA. Plates were coated with monoclonal anti–I-309 antibodies and incubated with serial dilutions of the supernatants. Bound I-309 was detected with biotinylated secondary I-309 antibodies. I-309 concentrations were determined using recombinant human I-309 as a standard. The columns show the mean ± SD of 3 independent experiments.

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