Constitutive MPO and enhanced Mac-1α expression in 32D/ε cells.

(A) MPO mRNA expression in 32Dcl3 cells. The blot shown in Figure 1C was reprobed with MPO. (B,C) 32D/ε cells cultured in IL-3 were washed twice with PBS and then stimulated with 50 ng/mL G-CSF. Cells were harvested at the times indicated and subjected to Northern blot analysis (B). Expression of MPO was confirmed by immunohistochemical staining of 32Dcl3 and 32D/ε cells cultured in IL-3 or treated with 50 ng/mL G-CSF for 3 days (C; original magnification, ×400). (D) FACS analysis of Mac-1α in 32D and 32D/ε cells cultured in IL-3 or treated with 50 ng/mL G-CSF for 3 days.