Priming of 32D/ε cells for granulocyte differentiation.

Shown are the morphologic characteristics of parental 32Dcl3 cells and 32D/ε cells maintained in IL-3, treated with 50 ng/mL G-CSF for the period indicated (d4 indicates 4 days; and d7, 7 days), and cultured in medium without cytokines (no factor) for 4 days. The morphologic features of the cells were visualized using Wright-Giemsa staining (original magnification, ×400).