Immunodetection of hPBP in the transgenic mice platelets.

(A) SDS-PAGE gels of equal quantities of platelet protein were immunoblotted with an anti–NAP-2 polyclonal antibody that does not recognize mPBP proteins. Platelet proteins from the transgenic linesLong-PBP and λ-PBP/PF4 were studied. Control lanes include wild-type mice and 4 different normal human volunteers (a through d). (B) The relative expression of hPBP in the transgenic mice to the average value in the 4 human samples per milligram total platelet protein was determined and compared to its copy number. The dotted line is the average human platelet hPBP value. ●,Long-PBP; ♦, λ-PBP/PF4. Data are shown for average ± 1 SD. Experiments were repeated 3 separate times.