Flow cytometric analysis of Kit expression on wild-type Kit– and D816V-transfected Jurkat cells.

Transfection efficiency of D816V and wild-type Kit transfectants were compared with Jurkat cells transfected with the vector pcDNA-3 alone and the Kit-expressing cell line HMC-1.2. The upper panel represents cells stained with the IgG1 isotype control and the lower panel, cells stained with Kit monoclonal antibody. FSC indicates forward scatter. Numbers shown in each quadrant refer to the percentages of cells bearing surface Kit. Data are representative of 3 separate experiments.