Transendothelial migration.

(A) Supernatants (Sup) from CD40-stimulated tumor cells induce transendothelial migration of activated T cells. Supernatants obtained from cultures of leukemia cells from 3 representative patients were used at 100% vol/vol. Activated T cells were derived from 3 different donors (T-1 to T-3). Recombinant MDC and TARC were used at 500 ng/mL. Data are expressed as percentage of migrated cells. (B) The migration induced by supernatant of CD40-stimulated ALL cells is inhibited by disruption of chemokine gradient, by the addition of MDC, TARC, or supernatants to the Transwell upper chamber. Results represent mean ± SE of 3 individual experiments. Legend indicates the supernatant placed in the lower chamber followed by reagent placed in the upper chamber. (C) A combination of neutralizing anti-MDC and anti-TARC antibodies abrogates the migration induced by supernatants of CD40-stimulated ALL cells. Results represent mean ± SE of 3 individual experiments.