Fig. 7.
Fig. 7. Blocking of c-FLIPL–independent CHX-induced apoptosis by TECK. / (A) MOLT4 cells were stimulated with given treatments for 6 hours. Protein lysates were prepared, and Western blot analysis was conducted with the use of specific antibodies. For the detection of cleaved poly (adenosine 5′-diphosphate–ribose) polymerase (PARP), cleavage-specific antibody was used. (B) MOLT4 cells were treated with CHX at varied concentrations in the presence of TECK or Z-VAD-FMK for 8 hours. Western blots were performed with the use of anti-cleavage–specific PARP and anti–c-FLIPL. This is one of 2 reproducible experiments.

Blocking of c-FLIPL–independent CHX-induced apoptosis by TECK.

(A) MOLT4 cells were stimulated with given treatments for 6 hours. Protein lysates were prepared, and Western blot analysis was conducted with the use of specific antibodies. For the detection of cleaved poly (adenosine 5′-diphosphate–ribose) polymerase (PARP), cleavage-specific antibody was used. (B) MOLT4 cells were treated with CHX at varied concentrations in the presence of TECK or Z-VAD-FMK for 8 hours. Western blots were performed with the use of anti-cleavage–specific PARP and anti–c-FLIPL. This is one of 2 reproducible experiments.

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