Inhibition of chemotactic responsiveness of MOLT4 cells to TECK by wortmannin.

(A) MOLT4 cells were pretreated with varying concentrations of wortmannin (WM), PD98059, or pertussis toxin (PT) for 1 hour, washed twice with serum-free medium, and employed for transwell migration assay. (B) MOLT4 cells treated with DMSO or wortmannin (1 μM) were loaded with Fura-2AM and stimulated with 100 nM TECK. Calcium flux was monitored by measuring relative fluorescence of Fura-2AM. SDs were obtained from at least 3 independent experiments.