MTT assay of MM cells and healthy donor B cells cultured with human recombinant TRAIL.

Dose-response survival curves based on MTT colorimetric assay were generated for MM cells and healthy donor B cells cultured for 18 hours with TRAIL (10-1000 ng/mL). (A) Dose-response survival curves are shown for TRAIL-sensitive MM cell lines ARP-1; Dex-sensitive MM-1S and Dex-resistant MM-1R; RPMI-8226/S and its cytotoxic drug-resistant sublines Dox6, Dox40, MR20, and LR5; as well as the response of OCI-My5 cells, which have intermediate TRAIL sensitivity. (B) Dose-response survival curves are shown for TRAIL-resistant ARH-77, HS Sultan, and IM-9 MM cells, as well as healthy donor peripheral blood B cells.