GILZ inhibits nuclear traslocation of NF-κB.

(A) Immunofluorescence analysis of empty vector–transfected (PV6; i, ii, v, vi) or GILZ-transfected (GIRL19; iii, iv, vii, viii) clones. Cells untreated (i, iii, v, vii) or treated for 2 hours on plates coated with anti-CD3 (ii, iv, vi, viii) were stained with anti-p65 antibody (i, ii, iii, iv) or anti–NF-AT antibody (v, vi, vii, viii) by paraformaldehyde-saponin procedure. (B) Cytofluorimetric analysis of TCR-induced apoptosis in PV6 (i, untreated; ii, treated on plates coated with anti-CD3) and GIRL19 (iii, untreated; iv, treated with anti-CD3) clones as evaluated at 18 hours by PI assay. (C) Western blot analysis of GILZ protein in PV6 (lane 1) and GIRL19 (lane 2) clones. Original magnification: × 900.