GILZ inhibits p65/p52-dependent transactivation of a pGL3–IL-2 reporter vector.

Analysis of transcriptional activity was performed on 293 cells transiently transfected with a luciferase reporter gene (pGL3–IL-2) containing an IL-2 promoter region. The indicated amounts of PMT2T-GILZ, PMT2T-p65, and PMT2T-p52, alone or in combination, were cotransfected with 15 μg of the IL-2–driven luciferase plasmid (A-B) or control vector pRc/RSV-luc (C). The values are expressed as fold increases of luciferase activity. Each transfection was performed in triplicate, and SD bars are shown. EC indicates endogenous control.