Chelator 311 markedly reduced the expression of cyclin-dependent kinase 2 (cdk-2) but not cdk4.

The SK-N-MC neuroepithelioma cell line was incubated for 30 hours at 37°C with DFO (5-150 μM) or 311 (1-25 μM). Western blot analysis was then performed as described in “Materials and methods.” Results shown are representative of 3 separate experiments performed.