Fig. 3.
Inhibition of FLT3 phosphorylation by AG1295.
Blasts from an AML sample with constitutively activated FLT3 were incubated at 37°C and 5% CO2 for 4 hours with increasing concentrations of AG1295. Clarified lysates (500 μg) were immunoprecipitated (IP) with anti-FLT3 antibody, resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to membranes, immunoblotted (IB) with antiphosphotyrosine (p-tyr) or anti-FLT3 antibodies, and visualized with chemiluminescence. Arrowheads identify FLT3.
