Epitope mapping using canine-human chimeras.

(A) Schematic overview of the different canine-human chimeras used for epitope mapping. (B) Scheme of the N-terminal region of GPIbα and localization of the epitopes of several anti-GPIb mAbs. Monoclonal antibodies given are those screened in this article (bold, group 1 mAbs; italic, group 2 mAbs) and those described by Shen et al15 (regular, not bold). Point mutations resulting in PT-vWD gain-of-function mutations (233, 239) are also indicated.