Quantification of liver and spleen L ferritin mRNA by RNAse protection assay.

Five micrograms of total RNA extracted from mouse tissues was hybridized with 5 × 10⁴ cpm of a GAPDH probe and 5 × 10⁴ cpm of an L ferritin probe. (A) Autoradiogram of a typical experiment. The protected fragments for L ferritin mRNA (LFt) and for GAPDH are shown for the liver and spleen of 2Fth^+/+ and 2 Fth^+/−mice. (B) Intensities of the protected fragments were quantified by Instantimager. Results are the mean of 2 independent experiments performed on 6 mice of each genotype. They are expressed as the ratio of radioactivity associated with each protected fragment. M indicates DNA markers. Sizes are indicated in bases.