Analysis of transgene expression in peripheral blood cells.

(A-D) Photomicrographs of circulating peripheral blood cells from transplant recipients expressing CGPMAPΔBel-encoded AP. Staining with Vector Red and hematoxylin shows an AP⁺ neutrophil (A) and an AP⁺ lymphocyte (B) with pink-stained cytoplasm. Staining with X-Phos shows darkly stained AP⁺ RBCs (C) and an AP⁺ WBC (large arrow) and AP⁺ platelet (small arrow) present in a smear examined by Nomarski microscopy (D). Original magnification 400 × in panels A-D. (E) Examples of flow cytometry data from the peripheral blood of a transplant recipient expressing CGPMscvF-encoded GFP (green lines) and an untransduced control animal (black lines), with the percentage of GFP⁺ cells indicated.