Flow cytometry analysis of IgG-bearing B cells with labeled PL vesicles.

The percentage of double-positive cells is expressed as the percentage of IgG-binding B cells and is indicated in the upper right quadrant of each panel. (A) PBMCs from patient CIC: binding of labeled CL vesicles. (B) PBMCs from a healthy donor: binding of labeled CL vesicles. (C) PBMCs from patient CIC: binding of PC vesicles. (D-F) PBMCs from patient CIC: inhibition of labeled CL vesicle binding with, respectively, nonlabeled CL vesicles, nonlabeled PC vesicles, or antihuman Fab Ab. Single-cell sorting was performed in the gate of the double-stained population shown in the upper right quadrant in panel A. IgG-PE indicates IgG conjugated with R-phycoerythrin.