Fig. 1.
Fig. 1. Strategies for V region amplifications and cloning. / VH and Vκ gene fragments were amplified by a single-cell PCR technique using a seminested PCR as second reaction (A,C). Four sets of PCR were necessary for the 5′ extension of VH regions (B), and 2 sets of PCR were required for the 5′ extension of Vκ regions (D). Gene fragments were cloned in a forced orientation (B,D). *Unique restriction cloning sites; Pep sig indicates signal peptide; polyh, polyhedrine promoter; p10, p10 promoter.

Strategies for V region amplifications and cloning.

VH and Vκ gene fragments were amplified by a single-cell PCR technique using a seminested PCR as second reaction (A,C). Four sets of PCR were necessary for the 5′ extension of VH regions (B), and 2 sets of PCR were required for the 5′ extension of Vκ regions (D). Gene fragments were cloned in a forced orientation (B,D). *Unique restriction cloning sites; Pep sig indicates signal peptide; polyh, polyhedrine promoter; p10, p10 promoter.

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