Fig. 5.
Comparison of STAT4 mRNA and STAT4 protein expression after IL-12 activation.
Activated T cells were cultured with cytokines indicated in the presence of anti-CD3 for 24 hours. Whole-cell extracts and total RNA were prepared. Northern blot was probed with RT-PCR–amplified STAT4 cDNA fragment (top panel), and the 28S ribosomal RNA in the RNA gel was used as quantity control (middle panel). Western blots were performed with whole-cell extracts and probed with anti-STAT4 (bottom panel).
