Fig. 2.
Mixing patient plasma with normal plasma generates TAT′ complex and impairs residual prothrombin activity.
(A) Mixtures of patient plasma (0%, ▪; 5%, ●; 10%, ▴; 50% final, ♦) and normal plasma were incubated at 37°C for 0 to 24 hours. The amount of TAT′ complex generated in each sample was measured by ELISA for normal TAT. (inset) TAT complex and TAT′ complex isolated from patient plasma were subjected to SDS-PAGE (10% separating gels under reducing conditions). (B) Patient plasma (0%, ▪; 5% final, ●) was mixed with normal plasma and incubated at 37°C for 0 to 24 hours. Residual prothrombin activity in each sample was measured.