Fig. 6.
![Fig. 6. MEK-1 dependency of VEGF-induced proliferation. / MM.1S cells were starved for 3 hours in RPMI 1640 with 5% FBS. Cells were incubated with 100 or 1000 ng/mL VEGF165 with or without PD98059 (25μM) or BIM (2μM). For the last 9 hours of 72-hour cultures, 0.5μCi [3H] thymidine was added, and radioactivity incorporated into DNA was determined by scintillation counting (Wallac Trilux Betaplate scintillation counter) of automatically harvested cells (Tomec Harvester 96 Mach III). Results are expressed as fold-proliferation of untreated cells (control). The data are means of 3 wells, and the experiment was repeated twice.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/2/10.1182_blood.v98.2.428/5/h81411288006.jpeg?Expires=1723738848&Signature=cf1j3XGxXJemkVFhccBY-TE5SZEm8xFzjdXppVbJhhF5GWw-fXkPNtT~WB-wCfxx9ip5IkEtx~7qQpWIo8Og6ZhqTuuhoLIgp-vqfwASDzcGU0jBfCI8aH~oBN3rZRAsco3Ycd8N~re6CXwv0DxOalh0W1RcCmtoZi7K0uT~RhktP6i-Kg5g6P5jaerbI5SNbY163f7~QlFOMTFTDklFcUqEsR0egzyvAb0wDz8-WZq5x5l0BgEedUnA5OvNaVyrCwy2VYlmiIpQCo4hhbba-RUAVe9SYuPU0eJu~BkZnuGUncvKAGKAKxPjJ~-sItR3zyBT8kPaXSbvaSr6nqNdmA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
MEK-1 dependency of VEGF-induced proliferation.
MM.1S cells were starved for 3 hours in RPMI 1640 with 5% FBS. Cells were incubated with 100 or 1000 ng/mL VEGF165 with or without PD98059 (25μM) or BIM (2μM). For the last 9 hours of 72-hour cultures, 0.5μCi [3H] thymidine was added, and radioactivity incorporated into DNA was determined by scintillation counting (Wallac Trilux Betaplate scintillation counter) of automatically harvested cells (Tomec Harvester 96 Mach III). Results are expressed as fold-proliferation of untreated cells (control). The data are means of 3 wells, and the experiment was repeated twice.
