Fig. 3.
Nicotine inhibits the formation of “cobblestone areas” in LTBMCs.
LTBMCs were treated with different concentrations of nicotine (10−5 to 10−8 M). Nicotine was added to the culture medium weekly during feedings, starting from day 0. Formation of the adherent layer was monitored under an inverted microscope. Photographs were taken after 1 week and 1 month of culture.
