Fig. 3.
![Fig. 3. Nonlinear relationship of fraction of sickle Hb and percentage of sickle RBCs. / A graph of the percent HbS as measured by HPLC versus a visual estimate of the percent sickle cell mouse–derived RBCs is compared to a predicted curve based on the difference in MCH between transgenic sickle cell mice (MHC = 9.3 pg/cell) and normal donor mice (MHC = 14.5 pg/cell). The predicted curve is based on the following formula: % Hemoglobin S = {[(% sickle RBCs)(9.3 pg/cell)] × 100}/{[(% sickle RBCs)(9.3 pg/cell)] + [(% normal RBCs)(14.5 pg/cell)]}.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/12/10.1182_blood.v97.12.3960/6/h81211164003.jpeg?Expires=1724238554&Signature=gR7RZ2nmABF9hQSuGnDMGO6u-X3wOyABS3IqF~mCf8IQLNHtcHOTDJP95g8yyRMqJ8370gLtL7XdQigTNYQHD8Y8S50l8kD-HQ2190C7JA14KQbXF0rJBev4eBUjufXuyHodEF466WuhKt~Km7oguBjt-Pnj4DyN7EuyNaWSd8cuLKxs~MDV-9mrwtdliNOcH8U8SscRoo9x~9bW34tc6zze~49crZdpI8DTOZU0Jxwy4SL3J1Car6EWUINEmB67eNtR3cuI6OgILm1i6qAAS7Yysi80evEQVJStuOBgducTV44u~p1AW~uQihV31yLvSAAdAFurUVnQQmjxaLosrw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Nonlinear relationship of fraction of sickle Hb and percentage of sickle RBCs.
A graph of the percent HbS as measured by HPLC versus a visual estimate of the percent sickle cell mouse–derived RBCs is compared to a predicted curve based on the difference in MCH between transgenic sickle cell mice (MHC = 9.3 pg/cell) and normal donor mice (MHC = 14.5 pg/cell). The predicted curve is based on the following formula: % Hemoglobin S = {[(% sickle RBCs)(9.3 pg/cell)] × 100}/{[(% sickle RBCs)(9.3 pg/cell)] + [(% normal RBCs)(14.5 pg/cell)]}.
