Fig. 5.
Fig. 5. Expression of C2GlcNAcT-I correlates with P-selectin ligands but not with E-selectin ligands on activated T cells. / Two-color analysis of 1B11 versus P-RIgM (A) or E-RIgM (B) in WT Th1 cells. Concordant staining is seen with P-RIgM, but not with E-RIgM, where 1B11+/E-RIgM− and 1B11−/E-RIgM+ subsets are also seen. (C, D) Analysis of C2GlcNAcT-I−/− Th1 cells as for panels A and B. As expected, no staining is seen for 1B11 or P-RIgM (C), but a clear subset of cells is E-RIgM+ but 1B11−(D).

Expression of C2GlcNAcT-I correlates with P-selectin ligands but not with E-selectin ligands on activated T cells.

Two-color analysis of 1B11 versus P-RIgM (A) or E-RIgM (B) in WT Th1 cells. Concordant staining is seen with P-RIgM, but not with E-RIgM, where 1B11+/E-RIgM and 1B11/E-RIgM+ subsets are also seen. (C, D) Analysis of C2GlcNAcT-I−/− Th1 cells as for panels A and B. As expected, no staining is seen for 1B11 or P-RIgM (C), but a clear subset of cells is E-RIgM+ but 1B11(D).

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