Protein levels and glycosylation isoforms ofEMP2.

(A) DAC and MV cells were compared for EMP2 protein expression by Western blot using an anti-EMP2 rabbit serum. DAC cells express significantly higher levels of EMP2 than MV cells. (B) Effect of tunicamycin treatment on EMP2 protein. Cells were incubated with tunicamycin, and a Western blot was performed using an anti-Flag M2 antibody. Bands I-III designate glycosylated EMP2 isoforms; Band IV represents the nonglycosylated protein with the predicted molecular weight of 20 kDa. (C) NIH3T3 cells infected with the pSRαEMP2 expression vector express 3 size isoforms ofEMP2.