American Society of Hematology

Fig. 1.

$Fig. 1. EMP2 has a discrete cell type and tissue distribution. / (A) Northern blot analysis of EMP2 in total DAC and MV RNA. The 4.8-kb EMP2 transcript was significantly higher in DAC than MV. rRNA bands indicate that the samples were equally loaded. (B) RT-PCR of EMP2 expression in NIH3T3 cells, peritoneal cells, Hardy fraction B cells, and plasma cells. PCR yielded the expected 514-bp EMP2 fragment in peritoneal and NIH3T3 fibroblasts. No EMP2 expression was detectable in Hardy fractions or plasma B cells. Glyceraldehyde phosphate dehydrogenase was used as a positive control to normalize expression between the different cell types. (C) EMP2 expression in murine tissue was evaluated by Northern dot blot probed with a 32P-labeledEMP2 fragment. Criteria for semiquantitative assessment of tissue expression are shown in the right panel; results are tabulated in the left panel.$

EMP2 has a discrete cell type and tissue distribution.

(A) Northern blot analysis of EMP2 in total DAC and MV RNA. The 4.8-kb EMP2 transcript was significantly higher in DAC than MV. rRNA bands indicate that the samples were equally loaded. (B) RT-PCR of EMP2 expression in NIH3T3 cells, peritoneal cells, Hardy fraction B cells, and plasma cells. PCR yielded the expected 514-bp EMP2 fragment in peritoneal and NIH3T3 fibroblasts. No EMP2 expression was detectable in Hardy fractions or plasma B cells. Glyceraldehyde phosphate dehydrogenase was used as a positive control to normalize expression between the different cell types. (C) EMP2 expression in murine tissue was evaluated by Northern dot blot probed with a ³²P-labeledEMP2 fragment. Criteria for semiquantitative assessment of tissue expression are shown in the right panel; results are tabulated in the left panel.

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