Fig. 5.
Fig. 5. MLR by B6 and B6-cdd T cells against BALB/c (host) alloantigens. / B6 (░) or B6-cdd (▪) spleen and lymph node CD4+ T cells were isolated and enriched by means of the Macs column. Then 20-Gy–irradiated BALB/c spleen cells or bone marrow–derived (granulocyte-macrophage colony-stimulating factor plus interleukin-4 (IL-4) dendritic cells (data not shown) were used to stimulate responses. The cultures were pulsed with [3H]thymidine for the final 24 hours, and cpm was measured after 96 hours. BALB/c dendritic cells also induced equivalent responses by B6-cdd– and B6–responding T cells (data not shown).

MLR by B6 and B6-cdd T cells against BALB/c (host) alloantigens.

B6 (░) or B6-cdd (▪) spleen and lymph node CD4+ T cells were isolated and enriched by means of the Macs column. Then 20-Gy–irradiated BALB/c spleen cells or bone marrow–derived (granulocyte-macrophage colony-stimulating factor plus interleukin-4 (IL-4) dendritic cells (data not shown) were used to stimulate responses. The cultures were pulsed with [3H]thymidine for the final 24 hours, and cpm was measured after 96 hours. BALB/c dendritic cells also induced equivalent responses by B6-cdd– and B6–responding T cells (data not shown).

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