HIF-1α protein and HIF target gene expression in Chinese hamster fibroblast mutant cell lines.
(A,C) Immunoblots showing levels of HIF-1α protein in cell extracts from respiration-deficient Chinese hamster fibroblast mutant cell lines. Cells were cultured in parallel for 4 hours in normoxia (21% O2, N) or hypoxia (0.1% O2, H). (A) Chinese hamster lung fibroblast wild-type CCL16 cells and the derived CCL16-B2 (B2) and CCL16-B9 (B9) cell lines. (B) RNAse protection assay showing expression of Glut-1 mRNA in wild-type CCL16 and CCL16-B2 (B2) cells. Cells were cultured in parallel for 16 hours in normoxia (21% O2, N) or hypoxia (0.1% O2, H). Signals from Glut-1 mRNA and U6 small nuclear RNA, as an internal control, are indicated. (C) Chinese hamster lung fibroblast wild-type V79 cells and the derived Gal32, V79-G4 (G4), and V79-G7 (G7) cell lines.
