Cleavage of hCAP-18 by exocytosed material after the immunoprecipitation of individual serine proteases.

Endogenous hCAP-18 fragments were deleted from the exocytosed material. Individual serine proteases were removed from the exocytosed material by immunoprecipitation before incubation with purified hCAP-18. Samples were run on SDS-PAGE followed by immunoblotting with anti–hCAP-18 antibodies. Purified hCAP-18 (lane a) was incubated with the exocytosed material after immunoprecipitation with preimmune rabbit antibodies (lane b), antielastase antibodies (lane c), anti–cathepsin G antibodies (lane d), and anti–proteinase 3 antibodies (lane e).