Fig. 3.
Immunoblotting of cell lysates.
Neutrophils were TCA-precipitated, and the precipitates were run on SDS-PAGE and analyzed by immunoblotting with anti–hCAP-18 antibodies. (A) Precipitates of neutrophils harvested from skin windows (107 cells/mL), unstimulated cells (lane a), or cells stimulated with fMLP (lane b), IgG-coated latex beads (lane c), or ionomycin (lane d). (B) Precipitates of neutrophils isolated from peripheral blood (107 cells/mL), unstimulated cells (lane a) or cells stimulated with fMLP (lane b), IgG-coated latex beads (lane c), or ionomycin (lane d).
