Fig. 4.
Phosphorylation of CPI and MBS at Thr-696 after stimulation of intact platelets with thrombin, ionomycin, or PMA.
Human platelets were stimulated with 0.1 U/mL thrombin, 0.5 μM ionomycin, or 50 nM PMA for the indicated time at 37°C under conditions of nonstirring, and whole platelet lysates originating from 30 μL of platelet suspension were resolved by SDS-PAGE. This was followed by immunoblotting with anti–pSer19-MLC20 and anti–pThr38–CPI-17 antibodies. Similar results were obtained in 3 other experiments using different donor platelets.
