![Fig. 7. IP3 production and calcium release are not observed in platelets stimulated with vWF plus botrocetin. / (A) IP3: after human platelets were stimulated with 75 μg/mL collagen (▪) or 6 μg/mL botrocetin plus 10 μg/mL vWF (▧) for the indicated time periods, the production of IP3was measured as described in “Materials and methods.” The data are expressed as means ± SEM of 3 separate experiments. (B) Ca++: stimulation of human platelets with 75 μg/mL collagen (i) or 6 μg/mL botrocetin plus 10 μg/mL vWF (ii) was initiated at the time indicated by the arrow. The [Ca++]i elevation in the absence of extracellular calcium was monitored as changes in fura2 fluorescence for 300 seconds. The ordinate represents the ratio of fura2 fluorescence. The results are representative of 3 experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/12/10.1182_blood.v97.12.3836/6/h81211191007.jpeg?Expires=1724148858&Signature=CFuF9x3Thbn7SOIjtVp0lf80IGLa8d2Z~A8Un8Yy87PUxo0MUeQw5CJe~gPde~sK0QlnXRKydvVFBdXKwYlBtXSFYwA1YQffGzZwiwFnbRW~CZ1sYpakbvhz1hzwGNjyAQONm-HsJt3VZT3sxRxSc19HbhKYduRIsMLrfHhtE87RdvoKDgXwSD2-LVP-N~6drYs4ta5gOt7f1tk8HJS4Zzm3qm9iRQr2PiFKPrzVCTXGaoIo3pSfs~CWm0ORlC3Pr6NxAobTPeFbOp7PZE~us8B2h~av5mGj~jBF175ZJehpkx-0ploGNLWAowfUXp80nhKPSbcj2q91fkSK2EN~oQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
IP3 production and calcium release are not observed in platelets stimulated with vWF plus botrocetin.
(A) IP3: after human platelets were stimulated with 75 μg/mL collagen (▪) or 6 μg/mL botrocetin plus 10 μg/mL vWF (▧) for the indicated time periods, the production of IP3was measured as described in “Materials and methods.” The data are expressed as means ± SEM of 3 separate experiments. (B) Ca++: stimulation of human platelets with 75 μg/mL collagen (i) or 6 μg/mL botrocetin plus 10 μg/mL vWF (ii) was initiated at the time indicated by the arrow. The [Ca++]i elevation in the absence of extracellular calcium was monitored as changes in fura2 fluorescence for 300 seconds. The ordinate represents the ratio of fura2 fluorescence. The results are representative of 3 experiments.
