Gene expression in cord blood progenitor subpopulations.
RNA was extracted and reverse-transcribed from FACS-isolated populations from pooled cord blood (lanes 1-4) or thymus (lanes 5 and 6), and cDNA from equal cell numbers of each population (600 cells for PU.1, Pax-5, and TdT; 1200 cells for IL-7Rα; 300 cells for β2-mgb) were subjected to PCR to amplify genes shown. Lane 1: CD34+CD38−CD7−, Lane 2: CD34+CD38−CD7+, Lane 3: B-lymphoid progenitors (CD34+CD19+), Lane 4: B cells (CD34−CD19+), Lane 5: CD3+CD4+CD8+ thymocytes, Lane 6: CD3+CD4+CD8− thymocytes, Lane 7: positive control cell lines (see “Materials and methods”), and Lane 8: negative control (no cDNA). Results are representative of at least 3 independent experiments. β2-mgb indicates β2-microglobulin.
